Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

CMV promoter mutants with a reduced propensity to productivity loss in CHO cells.

Scientific reports - 19 Nov 2015

Moritz Benjamin, Becker Peter B, Göpfert Ulrich

Abstract excerpt

The major immediate-early promoter and enhancer of the human cytomegalovirus (hCMV-MIE) is one of the most potent DNA elements driving recombinant gene expression in mammalian cells. Therefore, it is widely employed not only in research but also in large-scale industrial applications, e.g. for the production of therapeutic antibodies in Chinese hamster ovary cells (CHO). As we have reported previously, multi-site...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CHO Cells
Share to Clone Cells
Share to Cricetinae

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Cricetulus

Share to Cytomegalovirus

Share to Genes, Immediate-Early

Share to Promoter Regions, Genetic

Share to Recombinant Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 26581326
DOI: 10.1038/srep16952
PMCID: PMC4652263

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

CMV promoter mutants with a reduced propensity to productivity loss in CHO cells.PMID 26581326Read ↗

Insertion of core <scp>CpG</scp> island element into human <scp>CMV</scp> promoter for enhancing recombinant protein expression stability in <scp>CHO</scp> cellsSemantic proximity Read ↗

Engineered and Natural Promoters and Chromatin‐Modifying Elements for Recombinant Protein Expression in CHO CellsSemantic proximity Read ↗

How to train your cell - Towards controlling phenotypes by harnessing the epigenome of Chinese hamster ovary production cell lines.Semantic proximity Read ↗

Role of the Proximal Enhancer of the Major Immediate-Early Promoter in Human Cytomegalovirus ReplicationSemantic proximity Read ↗

Non-permissive CHO cells; A rapid approach for purification of recombinant Herpes Simplex Virus-1Semantic proximity Read ↗

Improving transcriptional activity of human cytomegalovirus major immediate-early promoter by mutating NF-κB binding sites.Semantic proximity Read ↗

Evaluating regulatory elements of human cytomegalovirus major immediate early gene for enhancing transgene expression levels in CHO K1 and HEK293 cellsSemantic proximity Read ↗

Rapid Genetic Engineering of Human Cytomegalovirus by Using a Lambda Phage Linear Recombination System: Demonstration that pp28 (UL99) Is Essential for Production of Infectious VirusSemantic proximity Read ↗

Further studies on the role of the residue 890 cysteine to tyrosine mutation in the M70 primase ORF of the temperature-sensitive mutant (tsm5) of murine cytomegalovirus.Semantic proximity Read ↗

Strain variations in single amino acids of the 86-kilodalton human cytomegalovirus major immediate-early protein (IE2) affect its functional and biochemical properties: implications of dynamic protein conformation.Semantic proximity Read ↗

Cloning of the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome in Escherichia coli: a new approach for construction of HCMV mutants.Semantic proximity Read ↗

Impact of Different Promoters on Episomal Vectors Harbouring Characteristic Motifs of Matrix Attachment RegionsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.