Article
The core element of a CpG island protects avian sarcoma and leukosis virus-derived vectors from transcriptional silencing.
Journal of virology - 1 Aug 2008
Senigl Filip, Plachý Jirí, Hejnar Jirí
Abstract excerpt
Unmethylated CpG islands are known to keep adjacent promoters transcriptionally active. In the CpG island adjacent to the adenosine phosphoribosyltransferase gene, the protection against transcriptional silencing can be attributed to the short CpG-rich core element containing Sp1 binding sites. We report here the insertion of this CpG island core element, IE, into the long terminal repeat of a retroviral vector...
Topics
- Animals
- Avian Leukosis Virus
- Binding Sites
- Birds
- CpG Islands
- Flow Cytometry
- Gene Silencing
- Genes, Reporter
- Humans
- Models, Biological
- Mutation
- Rous sarcoma virus
- Sarcoma, Avian
- Sp1 Transcription Factor
- Terminal Repeat Sequences
- Transcription, Genetic