Article
Polymorphisms in the tumor necrosis factor receptor genes affect the expression levels of membrane-bound type I and type II receptors.
Mediators of inflammation - 1 Jan 2014
Sennikov Sergey V, Vasilyev Filipp F, Lopatnikova Julia A, Shkaruba Nadezhda S, Silkov Alexander N
Abstract excerpt
The level of TNF receptors on various cells of immune system and its association with the gene polymorphism were investigated. Determining the levels of membrane-bound TNFα receptors on peripheral blood mononuclear cells (PBMCs) was performed by flow cytometry using BD QuantiBRITE calibration particles. Soluble TNF α receptor (sTNFRs) levels were determined by ELISA and genotyping was determined by PCR-RFLP....
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