Article
The role of distant mutations and allosteric regulation on LovD active site dynamics.
Nature chemical biology - 1 Jun 2014
Jiménez-Osés Gonzalo, Osuna Sílvia, Gao Xue, Sawaya Michael R, Gilson Lynne, Collier Steven J, Huisman Gjalt W, Yeates Todd O, Tang Yi, Houk K N
Abstract excerpt
Natural enzymes have evolved to perform their cellular functions under complex selective pressures, which often require their catalytic activities to be regulated by other proteins. We contrasted a natural enzyme, LovD, which acts on a protein-bound (LovF) acyl substrate, with a laboratory-generated variant that was transformed by directed evolution to accept instead a small free acyl thioester and no longer...
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