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Article

Enhancement of the organic solvent-stability of the LST-03 lipase by directed evolution.

Biotechnology progress - 1 Jan 2000

Kawata Takuya, Ogino Hiroyasu

Abstract excerpt

LST-03 lipase from an organic solvent-tolerant Pseudomonas aeruginosa LST-03 has high stability and activity in the presence of various organic solvents. In this research, enhancement of organic solvent-stability of LST-03 lipase was attempted by directed evolution. The structural gene of the LST-03 lipase was amplified by the error prone-PCR method. Organic solvent-stability of the mutated lipases was assayed by...

Read the complete abstract on PubMed

Topics

Amino Acid Sequence
Cloning, Molecular
Directed Molecular Evolution
Enzyme Stability
Escherichia coli
Lipase
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Mutation

Organic Chemicals

Pseudomonas aeruginosa

Sequence Alignment

Solvents

Source

Identifiers

PMID: 19731302
DOI: 10.1002/btpr.264

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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