Article
Disentangling homeologous contigs in allo-tetraploid assembly: application to durum wheat.
BMC bioinformatics - 1 Jan 2013
Ranwez Vincent, Holtz Yan, Sarah Gautier, Ardisson Morgane, Santoni Sylvain, Glémin Sylvain, Tavaud-Pirra Muriel, David Jacques
Abstract excerpt
BACKGROUND: Using Next Generation Sequencing, SNP discovery is relatively easy on diploid species and still hampered in polyploid species by the confusion due to homeology. We develop HomeoSplitter; a fast and effective solution to split original contigs obtained by RNAseq into two homeologous sequences. It uses the differential expression of the two homeologous genes in the RNA. We verify that the new sequences...
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