Article
A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome.
Genome biology - 31 Jan 2015
Chapman Jarrod A, Mascher Martin, Buluç Aydın, Barry Kerrie, Georganas Evangelos, Session Adam, Strnadova Veronika, Jenkins Jerry, Sehgal Sunish, Oliker Leonid, Schmutz Jeremy, Yelick Katherine A, Scholz Uwe, Waugh Robbie, Poland Jesse A, Muehlbauer Gary J, Stein Nils, Rokhsar Daniel S
Abstract excerpt
Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive, de novo, a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat, Triticum aestivum, and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation...
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