Article
ConservedPrimers 2.0: a high-throughput pipeline for comparative genome referenced intron-flanking PCR primer design and its application in wheat SNP discovery.
BMC bioinformatics - 13 Oct 2009
You Frank M, Huo Naxin, Gu Yong Q, Lazo Gerard R, Dvorak Jan, Anderson Olin D
Abstract excerpt
BACKGROUND: In some genomic applications it is necessary to design large numbers of PCR primers in exons flanking one or several introns on the basis of orthologous gene sequences in related species. The primer pairs designed by this target gene approach are called "intron-flanking primers" or because they are located in exonic sequences which are usually conserved between related species, "conserved primers"....
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