Article
Sequence-based SNP genotyping in durum wheat.
Plant biotechnology journal - 1 Sept 2013
van Poecke Remco M P, Maccaferri Marco, Tang Jifeng, Truong Hoa T, Janssen Antoine, van Orsouw Nathalie J, Salvi Silvio, Sanguineti Maria C, Tuberosa Roberto, van der Vossen Edwin A G
Abstract excerpt
Marker development for marker-assisted selection in plant breeding is increasingly based on next-generation sequencing (NGS). However, marker development in crops with highly repetitive, complex genomes is still challenging. Here we applied sequence-based genotyping (SBG), which couples AFLP®-based complexity reduction to NGS, for de novo single nucleotide polymorphisms (SNP) marker discovery in and genotyping of...
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