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Article

Separating homeologs by phasing in the tetraploid wheat transcriptome.

Genome biology - 25 Jun 2013

Krasileva Ksenia V, Buffalo Vince, Bailey Paul, Pearce Stephen, Ayling Sarah, Tabbita Facundo, Soria Marcelo, Wang Shichen, Akhunov Eduard, Uauy Cristobal, Dubcovsky Jorge

Abstract excerpt

BACKGROUND: The high level of identity among duplicated homoeologous genomes in tetraploid pasta wheat presents substantial challenges for de novo transcriptome assembly. To solve this problem, we develop a specialized bioinformatics workflow that optimizes transcriptome assembly and separation of merged homoeologs. To evaluate our strategy, we sequence and assemble the transcriptome of one of the diploid...

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Identifiers

PMID: 23800085
DOI: 10.1186/gb-2013-14-6-r66
PMCID: PMC4053977

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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