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Article

A genetic screen based on in vivo RNA imaging reveals centrosome-independent mechanisms for localizing gurken transcripts in Drosophila.

G3 (Bethesda, Md.) - 16 Apr 2014

Hayashi Rippei, Wainwright S Mark, Liddell Sophie J, Pinchin Sheena M, Horswell Stuart, Ish-Horowicz David

Abstract excerpt

We have screened chromosome arm 3L for ethyl methanesulfonate-induced mutations that disrupt localization of fluorescently labeled gurken (grk) messenger (m)RNA, whose transport along microtubules establishes both major body axes of the developing Drosophila oocyte. Rapid identification of causative mutations by single-nucleotide polymorphism recombinational mapping and whole-genomic sequencing allowed us to...

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Identifiers

PMID: 24531791
DOI: 10.1534/g3.114.010462
PMCID: PMC4059244

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Source receipt: pubmed, retrieved 7 Sept 2026.

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