Article
Photocleavable linkage between genotype and phenotype for rapid and efficient recovery of nucleic acids encoding affinity-selected proteins.
Journal of biotechnology - 15 Sept 2007
Doi Nobuhide, Takashima Hideaki, Wada Aiko, Oishi Yuko, Nagano Tetsuya, Yanagawa Hiroshi
Abstract excerpt
In vitro display technologies, such as mRNA display and DNA display are powerful tools to screen peptides and proteins with desired functions from combinatorial libraries in the fields of directed protein evolution and proteomics. When screening combinatorial libraries of polypeptides (phenotype), each of which is displayed on its gene (genotype), the problem remains, how best to recover the genotype moiety whose...
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