Article
Detection of clinically relevant copy number alterations in oral cancer progression using multiplexed droplet digital PCR.
Scientific reports - 19 Sept 2017
Hughesman Curtis B, Lu X J David, Liu Kelly Y P, Zhu Yuqi, Towle Rebecca M, Haynes Charles, Poh Catherine F
Abstract excerpt
Copy number alterations (CNAs), a common genomic event during carcinogenesis, are known to affect a large fraction of the genome. Common recurrent gains or losses of specific chromosomal regions occur at frequencies that they may be considered distinctive features of tumoral cells. Here we introduce a novel multiplexed droplet digital PCR (ddPCR) assay capable of detecting recurrent CNAs that drive tumorigenesis...
Topics
- Comparative Genomic Hybridization
- DNA Copy Number Variations
- DNA, Neoplasm
- Female
- HeLa Cells
- Human papillomavirus 16
- Human papillomavirus 18
- Humans
- Male
- Mouth Neoplasms
- Multiplex Polymerase Chain Reaction
- Papillomavirus Infections
- Polymorphism, Single Nucleotide