Article
A modified TALEN-based strategy for rapidly and efficiently generating knockout mice for kidney development studies.
PloS one - 1 Jan 2014
Liu Yunhong, Lv Xiaoyan, Tan Ruizhi, Liu Tianming, Chen Tielin, Li Mi, Liu Yuhang, Nie Fang, Wang Xiaoyan, Zhou Puhui, Chen Mianzhi, Zhou Qin
Abstract excerpt
The transcription activator-like effector nucleases (TALENs) strategy has been widely used to delete and mutate genes in vitro. This strategy has begun to be used for in vivo systemic gene manipulation, but not in an organ-specific manner. In this study, we developed a modified, highly efficient TALEN strategy using a dual-fluorescence reporter. We used this modified strategy and, within 5 weeks, we successfully...
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