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Article

Screening fluorescent voltage indicators with spontaneously spiking HEK cells.

PloS one - 1 Jan 2013

Park Jeehae, Werley Christopher A, Venkatachalam Veena, Kralj Joel M, Dib-Hajj Sulayman D, Waxman Stephen G, Cohen Adam E

Abstract excerpt

Development of improved fluorescent voltage indicators is a key challenge in neuroscience, but progress has been hampered by the low throughput of patch-clamp characterization. We introduce a line of non-fluorescent HEK cells that stably express NaV 1.3 and KIR 2.1 and generate spontaneous electrical action potentials. These cells enable rapid, electrode-free screening of speed and sensitivity of voltage...

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Identifiers

PMID: 24391999
DOI: 10.1371/journal.pone.0085221
PMCID: PMC3877367

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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