Article
Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator.
Neuron - 17 May 2023
Abdelfattah Ahmed S, Zheng Jihong, Singh Amrita, Huang Yi-Chieh, Reep Daniel, Tsegaye Getahun, Tsang Arthur, Arthur Benjamin J, Rehorova Monika, Olson Carl V L, Shuai Yichun, Zhang Lixia, Fu Tian-Ming, Milkie Daniel E, Moya Maria V, Weber Timothy D, Lemire Andrew L, Baker Christopher A, Falco Natalie, Zheng Qinsi, Grimm Jonathan B, Yip Mighten C, Walpita Deepika, Chase Martin, Campagnola Luke, Murphy Gabe J, Wong Allan M, Forest Craig R, Mertz Jerome, Economo Michael N, Turner Glenn C, Koyama Minoru, Lin Bei-Jung, Betzig Eric, Novak Ondrej, Lavis Luke D, Svoboda Karel, Korff Wyatt, Chen Tsai-Wen, Schreiter Eric R, Hasseman Jeremy P, Kolb Ilya
Abstract excerpt
The ability to optically image cellular transmembrane voltages at millisecond-timescale resolutions can offer unprecedented insight into the function of living brains in behaving animals. Here, we present a point mutation that increases the sensitivity of Ace2 opsin-based voltage indicators. We use the mutation to develop Voltron2, an improved chemigeneic voltage indicator that has a 65% higher sensitivity to...
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