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Article

A large-scale allosteric transition in cytochrome P450 3A4 revealed by luminescence resonance energy transfer (LRET).

PloS one - 1 Jan 2013

Sineva Elena V, Rumfeldt Jessica A O, Halpert James R, Davydov Dmitri R

Abstract excerpt

Effector-induced allosteric transitions in cytochrome P450 3A4 (CYP3A4) were investigated by luminescence resonance energy transfer (LRET) between two SH-reactive probes attached to various pairs of distantly located cysteine residues, namely the double-cysteine mutants CYP3A4(C64/C468), CYP3A4(C377/C468) and CYP3A4(C64/C121). Successive equimolar labeling of these proteins with the phosphorescent probe...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 24376769
DOI: 10.1371/journal.pone.0083898
PMCID: PMC3871636

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