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Article

An A245T mutation conveys on cytochrome P450eryF the ability to oxidize alternative substrates.

The Journal of biological chemistry - 17 Nov 2000

Xiang H, Tschirret-Guth R A, Ortiz De Montellano P R

Abstract excerpt

Cytochrome P450(eryF) (CYP107A1), which hydroxylates deoxyerythronolide B in erythromycin biosynthesis, lacks the otherwise highly conserved threonine that is thought to promote O-O bond scission. The role of this threonine is satisfied in P450(eryF) by a substrate hydroxyl group, making deoxyerythronolide B the only acceptable substrate. As shown here, replacement of Ala(245) by a threonine enables the oxidation...

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PMID: 10956654
DOI: 10.1074/jbc.M005811200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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