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Article

Role of active site loop in coenzyme binding and flavin reduction in cytochrome P450 reductase.

Archives of biochemistry and biophysics - 15 Sept 2016

Mothersole Robert G, Meints Carla E, Louder Alex, Wolthers Kirsten R

Abstract excerpt

Cytochrome P450 reductase (CPR) contains a loop within the active site (comprising Asp(634), Ala(635), Arg(636) and Asn(637); human CPR numbering) that relocates upon NADPH binding. Repositioning of the loop triggers the reorientation of an FAD-shielding tryptophan (Trp(679)) to a partially stacked conformer, reducing the energy barrier for displacement of the residue by the NADPH nicotinamide ring: an essential...

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Identifiers

PMID: 27461959
DOI: 10.1016/j.abb.2016.07.018

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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