Evidence that the LRRK2 ROC domain Parkinson's disease-associated mutants A1442P and R1441C exhibit increased intracellular degradation.

Abstract excerpt

Mutations in the leucine-rich repeat kinase 2 (lrrk2) gene are the leading genetic cause of Parkinson's disease (PD). In characterizing the novel ROC domain mutant A1442P, we compared its steady-state protein levels, propensity to aggregate, and toxicity with the pathogenic R1441C mutant and wild-type (WT) LRRK2. Mutant (R1441C and A1442P) and WT LRRK2 fused to green fluorescent protein (GFP) and FLAG were...

Topics

• Amino Acids
• Analysis of Variance
• Cell Survival
• Cysteine Proteinase Inhibitors
• Flow Cytometry
• Gene Expression Regulation
• Green Fluorescent Proteins
• HEK293 Cells
• Humans
• Hydrogen Peroxide