Article
Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation.
BMC biotechnology - 12 May 2020
Bánfalvi Zsófia, Csákvári Edina, Villányi Vanda, Kondrák Mihály
Abstract excerpt
BACKGROUND: Gene editing using the CRISPR/Cas9 system has become a routinely applied method in several plant species. The most convenient gene delivery system is Agrobacterium-mediated gene transfer with antibiotic selection and stable genomic integration of transgenes, including Cas9. For elimination of transgenes in the segregating progeny, selfing is applied in many plant species. This approach, however,...
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