Article
A one-time inducible transposon to create knockout mutants in rice.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Charng Yuh-Chyang
Abstract excerpt
Use of a transposon is an efficient tagging tool for exploring the function of the gene it inserts into or is adjacent to. A few modifications have been applied to the native Ac transposon to allow it to transpose efficiently or spontaneously and stop quickly thereafter. Furthermore, locating the transposon between a constitutive plant promoter and a reporter gene, such as the firefly luciferase gene, allows for...
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