Article
Sensitive mutant detection by concentrating mutant DNA with allele-specific capture and its application to analysis of contaminated grains in rice.
Plant cell reports - 1 Jun 2018
Kohata Ryuya, Koitabashi Kosuke, Kitashiba Hiroyasu, Nishio Takeshi
Abstract excerpt
KEY MESSAGE: We developed a method for detection of mutants in a large number of plants, and found this method to be applicable to detection of a mutant allele at a concentration of 1/1000. Many techniques for SNP analysis have been developed, but most of these techniques are not so sensitive to be used for detection of mutants in a large number of plants. Although some highly sensitive methods of SNP analysis...
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