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Article

Genetic selection scheme for isolation of signal transduction pathway mutants.

Applied and environmental microbiology - 1 Feb 2004

Anandan Shivanthi, Uram Jennifer

Abstract excerpt

Genetic characterization of a signal transduction pathway requires the isolation of mutations in the pathway. Characterization of these mutated genes and their loci enumerates the components of the pathway and leads to an understanding of the role of each gene locus in the pathway under study. We have designed and developed a strategy based on resistance to the chemical flucytosine for the identification of...

Topics

Cyanobacteria
Cytosine Deaminase
Drug Resistance, Bacterial
Escherichia coli
Escherichia coli Proteins
Flucytosine
Gene Expression Regulation, Bacterial
Mutation
Photosystem II Protein Complex
Promoter Regions, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Selection, Genetic

Signal Transduction

Transcription, Genetic

Source

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Identifiers

PMID: 14766578
DOI: 10.1128/AEM.70.2.967-972.2004
PMCID: PMC348942

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