Article
The effects of polymorphisms on human gene targeting.
Nucleic acids research - 1 Mar 2014
Deyle David R, Li Li B, Ren Gaoying, Russell David W
Abstract excerpt
DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In addition, we show that homology arm polymorphisms can be used to direct allele-specific targeting...
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