Article
The contribution of homology arms to nuclease-assisted genome engineering.
Nucleic acids research - 27 Jul 2017
Baker Oliver, Tsurkan Sarah, Fu Jun, Klink Barbara, Rump Andreas, Obst Mandy, Kranz Andrea, Schröck Evelin, Anastassiadis Konstantinos, Stewart A Francis
Abstract excerpt
Designer nucleases like CRISPR/Cas9 enable fluent site-directed damage or small mutations in many genomes. Strategies for their use to achieve more complex tasks like regional exchanges for gene humanization or the establishment of conditional alleles are still emerging. To optimize Cas9-assisted targeting, we measured the relationship between targeting frequency and homology length in targeting constructs using...
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