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Article

An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells.

PloS one - 1 Jan 2013

Koh Esther Y C, Ho Steven C L, Mariati, Song Zhiwei, Bi Xuezhi, Bardor Muriel, Yang Yuansheng

Abstract excerpt

A set of mutated Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) elements with varying strengths is generated by mutating the translation initiation codons of 10(th), 11(th), and 12(th) AUG to non-AUG triplets. They are able to control the relative expression of multiple genes over a wide range in mammalian cells in both transient and stable transfections. The relative strength of each IRES...

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Identifiers

PMID: 24349195
DOI: 10.1371/journal.pone.0082100
PMCID: PMC3857217

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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