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Article

Translational Efficiency of EMCV IRES in Bicistronic Vectors is Dependent Upon IRES Sequence and Gene Location

1 Sept 2006

Abstract excerpt

The internal ribosomal entry site (IRES)from encephalomyocarditis virus (EMCV) is a popular RNA element used widely in experimental and pharmaceutical applications to express proteins in eukaryotic cells or cell-free extracts. Inclusion of the wild-type element in monocistronic or bicistronic messenger RNAs (mRNAs) confers a high level of cap-independent translation activity to appropriately configured cistrons....

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PMID: 16989088
DOI: 10.2144/000112243

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