Article
Full-length novel MHC class I allele discovery by next-generation sequencing: two platforms are better than one.
Immunogenetics - 1 Jan 2014
Dudley Dawn M, Karl Julie A, Creager Hannah M, Bohn Patrick S, Wiseman Roger W, O'Connor David H
Abstract excerpt
Deep sequencing has revolutionized major histocompatibility complex (MHC) class I analysis of nonhuman primates by enabling high-throughput, economical, and comprehensive genotyping. Full-length MHC class I cDNA sequences, which are required to generate reagents such as MHC-peptide tetramers, cannot be directly obtained by short read deep sequencing. We combined data from two next-generation sequencing platforms...
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