Article
Major histocompatibility complex genotyping with massively parallel pyrosequencing.
Nature medicine - 1 Nov 2009
Wiseman Roger W, Karl Julie A, Bimber Benjamin N, O'Leary Claire E, Lank Simon M, Tuscher Jennifer J, Detmer Ann M, Bouffard Pascal, Levenkova Natalya, Turcotte Cynthia L, Szekeres Edward, Wright Chris, Harkins Timothy, O'Connor David H
Abstract excerpt
Major histocompatibility complex (MHC) genetics dictate adaptive cellular immune responses, making robust MHC genotyping methods essential for studies of infectious disease, vaccine development and transplantation. Nonhuman primates provide essential preclinical models for these areas of biomedical research. Unfortunately, given the unparalleled complexity of macaque MHCs, existing methodologies are inadequate...
Topics
- Animals
- Base Sequence
- DNA
- Genotype
- Histocompatibility Antigens Class I
- Macaca fascicularis
- Macaca mulatta
- Macaca nemestrina
- Molecular Sequence Data
- Polymorphism, Genetic
- Sequence Homology, Amino Acid