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No assembly required: Full-length MHC class I allele discovery by PacBio circular consensus sequencing.

Human immunology - 1 Dec 2015

Westbrook Catherine J, Karl Julie A, Wiseman Roger W, Mate Suzanne, Koroleva Galina, Garcia Karla, Sanchez-Lockhart Mariano, O'Connor David H, Palacios Gustavo

Abstract excerpt

Single-molecule real-time (SMRT) sequencing technology with the Pacific Biosciences (PacBio) RS II platform offers the potential to obtain full-length coding regions (∼1100-bp) from MHC class I cDNAs. Despite the relatively high error rate associated with SMRT technology, high quality sequences can be obtained by circular consensus sequencing (CCS) due to the random nature of the error profile. In the present...

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PMID: 26028281
DOI: 10.1016/j.humimm.2015.03.022

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CCS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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