Article
Rapid detection of pyrazinamide resistant Mycobacterium tuberculosis by high resolution melting curve analysis.
Journal of the Medical Association of Thailand = Chotmaihet thangphaet - 1 Sept 2013
Watcharasamphankul Worawan, Houpt Eric R, Foongladda Suporn
Abstract excerpt
OBJECTIVE: To develop a high-resolution melting curve analysis (HRM) assay for detection of PZA resistance. MATERIAL AND METHOD: Thirty samples of PZA-susceptible M. tuberculosis and eight isolates of PZA-resistant M. tuberculosis were included in the experiment. Five sets of primers were designed to cover the pncA gene and its upstream nucleotides. The pncA gene fragments were amplified by the PCR method....
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