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Article

Detection of Mutations in Mycobacterium tuberculosis pncA Gene by Modified High-Resolution Melting Curve Analysis of PCR Products.

Bulletin of experimental biology and medicine - 1 Dec 2019

Filipenko M L, Dymova M A, Cherednichenko A G, Khrapov E A, Mishukova O V, Schwartz Ya Sh

Abstract excerpt

We developed a protocol for detection of mutations in the pncA gene associated with M. tuberculosis resistance to pyrazinamide by analyzing melting curves of 7 overlapping amplicons with artificial heteroduplex formation (H-HRM) formed by co-amplification of wild-type DNA and test DNA and compared its efficiency and robustness with those of classical HRM analysis. Using HRM and H-HRM, we analyzed 35 PZAR DNA...

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Identifiers

PMID: 31782002
DOI: 10.1007/s10517-019-04688-6

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