Article
Characterization and mapping of the pyrophosphorolytic activity of the phage phi 29 DNA polymerase. Involvement of amino acid motifs highly conserved in alpha-like DNA polymerases.
The Journal of biological chemistry - 25 Apr 1991
Blasco M A, Bernad A, Blanco L, Salas M
Abstract excerpt
The phi 29 DNA polymerase, an alpha-like DNA polymerase, shows an inorganic pyrophosphate-dependent degradative activity with similar requirements to the corresponding one of Escherichia coli DNA polymerase I: (a) it requires a high concentration of inorganic pyrophosphate and is reversed by poly...
Topics
- Bacteriophages
- Base Sequence
- Catalysis
- DNA, Viral
- DNA-Directed DNA Polymerase
- Diphosphates
- Hydrolysis
- Molecular Sequence Data
- Mutation
- Substrate Specificity
- Templates, Genetic