Article
High-level expression, purification and characterization of a constitutively active thromboxane A2 receptor polymorphic variant.
PloS one - 1 Jan 2013
Xu Bing, Chakraborty Raja, Eilers Markus, Dakshinamurti Shyamala, O'Neil Joe D, Smith Steven O, Bhullar Rajinder P, Chelikani Prashen
Abstract excerpt
G protein-coupled receptors (GPCRs) exhibit some level of basal signaling even in the absence of a bound agonist. This basal or constitutive signaling can have important pathophysiological roles. In the past few years, a number of high resolution crystal structures of GPCRs have been reported, including two crystal structures of constitutively active mutants (CAM) of the dim-light receptor, rhodopsin. The...
Topics
- Amino Acid Sequence
- Detergents
- Gene Expression
- Genetic Engineering
- HEK293 Cells
- Humans
- Molecular Sequence Data
- Mutation
- Polymorphism, Genetic
- Protein Structure, Secondary
- Receptors, Thromboxane A2, Prostaglandin H2
- Tetracycline