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Article

The active site of TthPolX is adapted to prevent 8-oxo-dGTP misincorporation.

Nucleic acids research - 1 Jan 2014

Garrido Patricia, Mejia Edison, Garcia-Diaz Miguel, Blanco Luis, Picher Angel J

Abstract excerpt

Full genome sequencing of bacterial genomes has revealed the presence of numerous genes encoding family X DNA polymerases. These enzymes play a variety of biological roles and, accordingly, display often striking functional differences. Here we report that the PolX from the heat-stable organism Thermus thermophilus (TthPolX) inserts the four dNTPs with strong asymmetry. We demonstrate that this behaviour is...

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PMID: 24084083
DOI: 10.1093/nar/gkt870
PMCID: PMC3874185

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