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Article

Cloning and expression of a structural gene from Chlorobium vibrioforme that complements the hemA mutation in Escherichia coli.

Journal of bacteriology - 1 Mar 1990

Avissar Y J, Beale S I

Abstract excerpt

Escherichia coli SASX41B carries the hemA mutation and requires delta-aminolevulinic acid for growth. Strain SASX41B was transformed to prototrophy with pYA1, a plasmid vector carrying a 5.8-kilobase insert of genomic DNA from the green sulfur bacterium Chlorobium vibrioforme. Cell extracts prepared from transformed cells are able to catalyze transfer of label from [1-14C]glutamate or [3,4-3H]glutamyl-tRNA to...

Topics

Aminolevulinic Acid
Chromatiaceae
Cloning, Molecular
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Genetic Complementation Test
Genotype
Levulinic Acids
Mutation
Restriction Mapping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 2407729
DOI: 10.1128/jb.172.3.1656-1659.1990
PMCID: PMC208645

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2407729-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Cloning and expression of a structural gene from Chlorobium vibrioforme that complements the hemA mutation in Escherichia coli.PMID 2407729Read ↗

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