Article
Cloning of the gene for Escherichia coli glutamyl-tRNA synthetase.
Gene - 1 Jan 2000
Sanfaçon H, Levasseur S, Roy P H, Lapointe J
Abstract excerpt
The structural gene for the glutamyl-tRNA synthetase of Escherichia coli has been cloned in E. coli strain JP1449, a thermosensitive mutant altered in this enzyme. Ampicillin-resistant and tetracycline-sensitive thermoresistant colonies were selected following the transformation of JP1449 by a bank of hybrid plasmids containing fragments from a partial Sau3A digest of chromosomal DNA inserted into the BamHI site...
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