Article
Cloning of the gene for Escherichia coli glutamyl-tRNA synthetase.
Gene - 1 Jan 2000
Sanfaçon H, Levasseur S, Roy P H, Lapointe J
Abstract excerpt
The structural gene for the glutamyl-tRNA synthetase of Escherichia coli has been cloned in E. coli strain JP1449, a thermosensitive mutant altered in this enzyme. Ampicillin-resistant and tetracycline-sensitive thermoresistant colonies were selected following the transformation of JP1449 by a ba...
Topics
- Amino Acyl-tRNA Synthetases
- Anti-Bacterial Agents
- Base Sequence
- Cloning, Molecular
- DNA Restriction Enzymes
- Drug Resistance, Microbial
- Escherichia coli
- Genes
- Genes, Bacterial
- Glutamate-tRNA Ligase
- Mutation
- Nucleic Acid Hybridization
