Article
Characterization of the natural deletion mutant of plasmid pXZ10145 in Corynebacterium glutamicum and construction of a recombinant plasmid.
Chinese journal of biotechnology - 1 Jan 1991
Na S, Shen T, Jia P, Men D, Chen Q
Abstract excerpt
Plasmid pNAT65, carrying the chloramphenicol resistance marker, was chosen from a number of natural deletion mutants of pXZ10145 when pXZ10145 DNA, originally isolated from Corynebacterium glutamicum 1014-6T, was used to transform the protoplast of Corynebacterium crenatum T6-13. The size of pNAT65 was 2.4 kb, determined by electrophoresis on 0.7% agarose gel. The physical map of plasmid pNAT65 was determined....
Topics
- Chromosome Deletion
- Corynebacterium
- DNA, Recombinant
- Mutation
- Plasmids
- Protoplasts
- Restriction Mapping
- Transformation, Genetic