Article
Partial inhibition of protein synthesis accelerates the synthesis of porphyrin in heme-deficient mutants of Escherichia coli.
Molecular & general genetics : MGG - 15 Nov 1995
Nakayashiki T, Nishimura K, Tanaka R, Inokuchi H
Abstract excerpt
Mutants of Escherichia coli defective in the HemA protein grow extremely poorly as the result of heme deficiency. A novel hemA mutant was identified whose rate of growth was dramatically enhanced by addition to the medium of low concentrations of translational inhibitors, such as chloramphenicol...
Topics
- Aldehyde Oxidoreductases
- Amino Acid Sequence
- Base Sequence
- Chloramphenicol
- Cloning, Molecular
- DNA Primers
- Escherichia coli
- Genes, Bacterial
- Genotype
- Molecular Sequence Data
- Point Mutation
- Polymerase Chain Reaction
- Porphyrins
- Protein Synthesis Inhibitors
- Recombinant Proteins
- Sequence Homology, Amino Acid
- Tetracycline
- beta-Galactosidase