Article
Cloning, mapping and characterization of the Pseudomonas aeruginosa hemL gene.
Molecular & general genetics : MGG - 21 Aug 1995
Hungerer C, Troup B, Römling U, Jahn D
Abstract excerpt
The rate-limiting step in the biosynthesis of tetrapyrroles is the formation of 5-aminolevulinic acid (ALA). In Pseudomonas aeruginosa ALA is synthesized via a two-step reaction from aminoacylated tRNA(Glu) by the action of glutamyl-tRNA reductase and glutamate-1-semialdehyde-2,1-amino mutase. To initiate an investigation of the regulation of the second step in ALA formation, the hemL gene was cloned from P....
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- Environment
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genetic Complementation Test
- Intramolecular Transferases