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Article

Analysis of mutations that uncouple transport from phosphorylation in enzyme IIGlc of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system.

Journal of bacteriology - 1 May 1992

Ruijter G J, van Meurs G, Verwey M A, Postma P W, van Dam K

Abstract excerpt

Mutations that uncouple glucose transport from phosphorylation were isolated in plasmid-encoded Escherichia coli enzyme IIGlc of the phosphoenolpyruvate-dependent phosphotransferase system (PTS). The uncoupled enzymes IIGlc were able to transport glucose in the absence of the general phosphoryl-carrying proteins of the PTS, enzyme I and HPr, although with relatively low affinity. Km values of the uncoupled...

Topics

Base Sequence
Binding Sites
Biological Transport
Chromosome Mapping
Escherichia coli
Genes, Bacterial
Glucose
Kinetics
Mutation
Phosphoenolpyruvate Sugar Phosphotransferase System

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phosphorylation

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Identifiers

PMID: 1569016
DOI: 10.1128/jb.174.9.2843-2850.1992
PMCID: PMC205935

Genes

PTS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1569016-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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