Article
Cluster of point mutations predetermined by a quasipalindromic nucleotide sequence in plasmid pBR322 DNA.
FEBS letters - 12 Feb 1990
Salganik R I, Dianov G L, Medvedev O A
Abstract excerpt
Development of a cluster of point mutations due to the correction of an imperfect hairpin in plasmid DNA was investigated. Plasmid pBR322 DNA containing opposite double-strand DNA lesions in the region of a quasipalindrome was constructed. For this aim plasmid DNA was cleaved at the BamHI site, and cytosine residues of the sticky ends were modified by O-methylhydroxylamine. Modified linearized plasmid DNA was...
Topics
- Base Sequence
- DNA, Bacterial
- Deoxyribonuclease BamHI
- Deoxyribonucleases, Type II Site-Specific
- Escherichia coli
- Hydroxylamines
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plasmids
- Repetitive Sequences, Nucleic Acid