Article
Formation of intramolecular triplex in homopurine-homopyrimidine mirror repeats with point substitutions.
Nucleic acids research - 25 Nov 1990
Belotserkovskii B P, Veselkov A G, Filippov S A, Dobrynin V N, Mirkin S M, Frank-Kamenetskii M D
Abstract excerpt
We have used two-dimensional gel electrophoresis to study the structural transition to the triplex H form of sequences 5'-AAGGGAGAAXGGGGTATAGGGGYAAGAGGGAA-3' where X and Y are any DNA bases. The transition was observed at acid pH under superhelical stress. For X = Y = A or X = Y = G the sequences corresponded to homopurine-homopyrimidine mirror repeats (H-palindrome) which are known to adopt the H form under acid...
Topics
- Base Composition
- Base Sequence
- Cloning, Molecular
- DNA
- Electrophoresis, Gel, Two-Dimensional
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligonucleotides
- Plasmids