Article
Complementation of myosin null mutants in Dictyostelium discoideum by direct functional selection.
Developmental biology - 1 Feb 1990
Egelhoff T T, Manstein D J, Spudich J A
Abstract excerpt
The eukaryotic slime mold Dictyostelium discoideum contains a single conventional myosin heavy chain gene (mhcA). Cell lines in which this gene was deleted via homologous recombination have been previously reported. These myosin null cells were shown to be defective for cytokinesis and for sporogenesis. We demonstrate here that the cloned mhcA gene can be reintroduced into these cells by the use of a direct...
Topics
- Blotting, Southern
- Blotting, Western
- DNA, Fungal
- Dictyostelium
- Fluorescent Antibody Technique
- Genes, Fungal
- Genetic Complementation Test
- Mutation
- Myosin Subfragments
- Plasmids
- Promoter Regions, Genetic