Article
Genetically engineered truncated myosin in Dictyostelium: the carboxyl-terminal regulatory domain is not required for the developmental cycle.
Proceedings of the National Academy of Sciences of the United States of America - 1 Oct 1990
O'Halloran T J, Spudich J A
Abstract excerpt
The study of engineered Dictyostelium mutants with altered or missing myosin has revealed the molecule to be essential both for cytokinesis and for completion of the complex Dictyostelium developmental cycle. To explore the biological role of the carboxyl-terminal portion of the myosin tail, we have created a Dictyostelium cell line bearing a mutation designated my delta C34 in the myosin (mhcA) locus. This cell...
Topics
- Blotting, Southern
- Cell Cycle
- DNA, Fungal
- Dictyostelium
- Genetic Engineering
- Mutation
- Myosins
- Phenotype
- Plasmids
- Restriction Mapping