In vitro synthesis of selenocysteinyl-tRNA(UCA) from seryl-tRNA(UCA): involvement and characterization of the selD gene product.

doi:10.1073/pnas.87.2.543

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Article

In vitro synthesis of selenocysteinyl-tRNA(UCA) from seryl-tRNA(UCA): involvement and characterization of the selD gene product.

Proceedings of the National Academy of Sciences of the United States of America - 1 Jan 1990

Leinfelder W, Forchhammer K, Veprek B, Zehelein E, Böck A

Abstract excerpt

The selD gene from Escherichia coli, whose product is involved in selenium metabolism, has been cloned and sequenced. selD codes for a protein of 347 amino acids with a calculated molecular weight of 36,687. Analysis of the selD gene product through expression of the gene in the phage T7 promoter/polymerase system confirmed the predicted molecular weight of the protein. Gene disruption experiments demonstrated...

Topics

Amino Acid Sequence
Bacterial Proteins
Base Sequence
Codon
Drosophila Proteins
Escherichia coli
Genes, Bacterial
Genotype
Molecular Sequence Data
Mutation
Phosphotransferases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

RNA, Transfer, Amino Acid-Specific

RNA, Transfer, Amino Acyl

RNA, Transfer, Ser

Restriction Mapping

Selenium

Source

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Identifiers

PMID: 2405383
DOI: 10.1073/pnas.87.2.543
PMCID: PMC53301

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2405383-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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