Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells.

The Journal of cell biology - 1 Feb 1990

Masuda H, Hirano T, Yanagida M, Cande W Z

Abstract excerpt

To investigate the mechanisms of spindle elongation and chromosome separation in the fission yeast Schizosaccharomyces pombe, we have developed an in vitro assay using a temperature-sensitive mutant strain, nuc2. At the restrictive temperature, nuc2 cells are arrested at a metaphase-like stage with short spindles and condensed chromosomes. After permeabilization of spheroplasts of the arrested cells, spindle...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chromosomes, Fungal
Share to Fluorescent Antibody Technique
Share to Microtubules

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Mitosis

Share to Mutation

Share to Phenotype

Share to Schizosaccharomyces

Share to Temperature

Source

Open in PubMed Open full text

Identifiers

PMID: 2404993
DOI: 10.1083/jcb.110.2.417
PMCID: PMC2116017

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells.PMID 2404993Read ↗

The spindle pole bodies facilitate nuclear envelope division during closed mitosis in fission yeast.Semantic proximity Read ↗

Nuc2p, a subunit of the anaphase-promoting complex, inhibits septation initiation network following cytokinesis in fission yeast.Semantic proximity Read ↗

Fission yeast TPR-family protein nuc2 is required for G1-arrest upon nitrogen starvation and is an inhibitor of septum formation.Semantic proximity Read ↗

Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast.Semantic proximity Read ↗

A screen for genes involved in the anaphase proteolytic pathway identifies tsm1(+), a novel Schizosaccharomyces pombe gene important for microtubule integrity.Semantic proximity Read ↗

Bypassing anaphase by fission yeast cut9 mutation: requirement of cut9+ to initiate anaphase.Semantic proximity Read ↗

Astral microtubules are not required for anaphase B in Saccharomyces cerevisiae.Semantic proximity Read ↗

Fission yeast mal2+ is required for chromosome segregation.Semantic proximity Read ↗

Microtubule nucleation promoters Mto1 and Mto2 regulate cytokinesis in fission yeast.Semantic proximity Read ↗

Microtubules mediate mitochondrial distribution in fission yeast.Semantic proximity Read ↗

The interaction between Ran and NTF2 is required for cell cycle progression.Semantic proximity Read ↗

Assessment of mitotic spindle phenotypes in Drosophila S2 cells.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.