Article
In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells.
The Journal of cell biology - 1 Feb 1990
Masuda H, Hirano T, Yanagida M, Cande W Z
Abstract excerpt
To investigate the mechanisms of spindle elongation and chromosome separation in the fission yeast Schizosaccharomyces pombe, we have developed an in vitro assay using a temperature-sensitive mutant strain, nuc2. At the restrictive temperature, nuc2 cells are arrested at a metaphase-like stage wi...
Topics
- Adenylyl Imidodiphosphate
- Animals
- Cell Membrane Permeability
- Chromosomes, Fungal
- Fluorescent Antibody Technique
- Microtubules
- Mitosis
- Mutation
- Phenotype
- Saccharomycetales
- Schizosaccharomyces
- Spindle Apparatus
- Temperature
- Vanadates