Article
Visual DNA detection and SNP genotyping using asymmetric PCR and split DNA enzymes.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Neo Jia Ling, Uttamchandani Mahesh
Abstract excerpt
We describe a method to detect DNA sequences visually through a color change reaction using DNAzymes. We successfully applied the assay for the detection of Salmonella and Mycobacterium DNA, as well as for genotyping single base differences from within human genomic DNA samples. Our approach adopts a split probe targeting system, designed with G-rich sequences, which reassembles in the presence of target DNA,...
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