Article
Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies.
Nucleic acids research - 25 Feb 1990
Kwok S, Kellogg D E, McKinney N, Spasic D, Goda L, Levenson C, Sninsky J J
Abstract excerpt
We investigated the effects of various primer-template mismatches on DNA amplification of an HIV-1 gag region by the polymerase chain reaction (PCR). Single internal mismatches had no significant effect on PCR product yield while those at the 3'-terminal base had varied effects. A:G, G:A, and C:C mismatches reduced overall PCR product yield about 100-fold, A:A mismatches about 20-fold. All other 3'-terminal...
Topics
- Base Composition
- Base Sequence
- Gene Products, gag
- Genes, Viral
- HIV-1
- Molecular Sequence Data
- Mutation
- Nucleic Acid Amplification Techniques
- Oligonucleotide Probes
- Polymerase Chain Reaction