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Article

Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies.

Nucleic acids research - 25 Feb 1990

Kwok S, Kellogg D E, McKinney N, Spasic D, Goda L, Levenson C, Sninsky J J

Abstract excerpt

We investigated the effects of various primer-template mismatches on DNA amplification of an HIV-1 gag region by the polymerase chain reaction (PCR). Single internal mismatches had no significant effect on PCR product yield while those at the 3'-terminal base had varied effects. A:G, G:A, and C:C mismatches reduced overall PCR product yield about 100-fold, A:A mismatches about 20-fold. All other 3'-terminal...

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Identifiers

PMID: 2179874
DOI: 10.1093/nar/18.4.999
PMCID: PMC330356

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Source receipt: pubmed, retrieved 7 Sept 2026.

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